Modifications to ribonucleic acids (RNAs) are integrally involved in the  function, integrity, metabolism, and biosynthesis of RNA molecules. There are  over 100 RNA modifications that have been discovered with the majority occurri -ng in transfer RNA (tRNA), but they can also be found in ribosomal RNA  (rRNA), small nuclear RNA (snRNA), messenger (mRNA), and long non-coding  RNA (ncRNA). Urinary excretion of modified nucleosides has been linked to a  variety of cancers, AIDS, and other malignant disease states. 1-methyladenosine (m1 A) is a prominent reversible post-transcriptional RNA modification that influences the function and structure of tRNA and rRNA. 1- Methyladenosine occurs at positions 9, 14, and 58 of tRNA, with position 58  (m1 A58) being important to tRNA stabilization.Methyltransferases catalyze the  addition of a methyl group to the first position nitrogen on the adenosine base stru cture, resulting in the addition of a positive charge to the molecule. The reaction is  reversible with demethylases such as ALKBH3. 1-methyladenosine can affect ri bosomal biogenesis, antibiotic resistance in bacteria, react to environmental stress in tRNA, and interfere with Watson-Crick base pairing, which ultimately affects  reverse transcription and protein translation. Urine samples of cancer, rheumatoid arthritis, and AIDS patients have all  demonstrated high levels of m1 A, which supports its role as a functional  detection biomarker. Elevated serum levels of 1-methyladenosine have been  detected under stress conditions. Recent 1-methyladenosine research is  uncovering new roles and mechanisms involving 1-methyladenosine.

While m1 dA in DNA is considered a form of damage leading to genomic mutations, and thus requiring repair, endogenous enzymes proliferate the m1 A  modification in RNA. The m1A at position 9 (m1 A9) is known to stabilize the  canonical cloverleaf motif in tRNA. In addition, the m1 A58 of tRNA is present in  all eukaryotic types which may imply it is vital to molecular structure and stability . While 1-methyladenosine has been identified as an early malignant disease  marker, its complete role as a modified nucleoside has yet to be elucidated. The 1-Methyladenosine (m1 A) ELISA Kit is a competitive enzyme immunoa ssay developed for rapid detection and quantitation of 1-methyladenosine in urine,  serum, or plasma samples. The quantity of 1-methyladenosine in unknown sample s is determined by comparing its absorbance with that of a known 1-methyladenosi ne standard curve. The kit has a 1-methyladenosine detection sensitivity of approxi mately 2 ng/mL. Each kit provides sufficient reagents to perform up to 96 assays,  including standard curve and unknown samples.

Figure 1: 1-Methyladenosine(m1A)

試劑盒組成：

Box 1 (shipped at room temperature): 

1. 96-well Protein Binding Plate (Part No. 231001):  One 96-well strip plate. 

2. Anti-1-Methyladenosine Antibody (Part No. 50991C):  One 10 µL vial of anti-1-Methyladenosine. 

3. Secondary Antibody, HRP Conjugate (1000X) (Part No. 230003):  One 20 µL vial. 

4. Assay Diluent (Part No. 310804): One 50 mL bottle. 

5. 10X Wash Buffer (Part No. 310806): One 100 mL bottle. 

6. Substrate Solution (Part No. 310807): One 12 mL amber bottle. 

7. Stop Solution (Part. No. 310808): One 12 mL bottle. 8. 1-Methyladenosine Standard (Part No. 50992C):  One 10 µL vial of 5 mg/mL 1-Methyladenosine in DMSO. 

Box 2 (shipped on blue ice packs) 

1. 1-Methyladenosine Conjugate (1000X) (Part No. 50993D): One 10 µL vial of 1- Methyladenosine-BSA conjugate in PBS. 

存儲條件：

Upon receipt, aliquot and store the Anti-1-Methyladenosine Antibody and 1-Methyladenosine Standard at -20ºC and the 1- Methyladenosine Conjugate (1000X) at -80ºC to avoid multiple  freeze/thaw cycles. 

Store all other components at 4ºC.

*本品僅供科(kē)研使用，請勿用于臨床與診斷。

說明書下載：1-Methyladenosine (m1A) ELISA Kit (Colorimetric).pdf