N6 -methyl-adenosine (m6 A) is the most common and abundant modification on RNA molecules present in eukaryotes. The m6 A modification is catalyzed by a methyltransferase complex METTL3 and removed by the recently discovered m6 A RNA demethylases FTO and ALKBH5, which catalyze m6 A demethylation in an α-ketoglutarate (α-KG)- and Fe2+-dependent manner. It was shown that METTL3, FTO and ALKBH5 play important roles in many biological processes, ranging from development and metabolism to fertility. m6 A accounts for more than 80% of all RNA base methylations and exists in various species. m6 A is mainly distributed in mRNA and also occurs in noncoding RNA such as tRNA, rRNA and snRNA. The relative abundance of m6 A in mRNA transcripts has been shown to affect RNA metabolism processes such as splicing, nuclear export, translation ability and stability and RNA transcription. Abnormal m6 A methylation levels induced by defects in m6 A RNA methylase and demethylase could lead to dysfunction of RNA and cause disease.
For example, abnormally low levels of m6 A in target mRNAs due to increased FTO activity in patients with FTO mutations, through an as-yet undefined pathway, contributes to the onset of obesity and related diseases. The dynamic and reversible chemical m6 A modification on RNA may also serve as a novel epigenetic marker of profound biological significance. Therefore, more useful information for better understanding of m6 A RNA methylation levels and distribution on RNA transcripts could benefit diagnostics and therapeutics of disease.
Reversible m6 A methylation in mRNA
(Niu Y et al: Genomics, Proteomics & Bioinformatics, 11: 2013)
産品名稱 |
産品貨号 |
規格 |
m6A RNA Methylation Quantification Kit(Colorimetric) |
GK-4048 |
96assays |
試劑盒組成:
*在使用之前将溶液離(lí)心至管底。
實驗過程:
産品優點:
● 極速:檢測方便快速。整個操作步驟僅需 3 小時 45 分鍾。
● 高(gāo)敏:檢測的(de)最低(dī)下限為(wèi) 10 pg 的(de) m6 A 。
● 特異性好:獨特的(de)結合液容許 >70 nts 的(de)RNA 緊緊的(de)結合在孔上,可(kě)以定量檢測來自(zì)mRNA和(hé)ncRNA如(rú)tRNA,rRNA 和(hé)snRNA。
● 專一(yī)性強:優化的(de)抗體和(hé)增強劑溶液可(kě)以特異的(de)定量檢測 m6 A,對于特定片段腺苷濃度範圍的(de)未甲基化樣本RNA 不會産生交叉反應。
● 靈活性好:96 孔可(kě)拆卸闆模式使研究人員能根據自(zì)己需要選擇手工或是高(gāo)通量模式分析。
● 可(kě)信:操作簡便、可(kě)信、分析 條件始終如(rú)一(yī)。
*本品僅供科(kē)研使用,請勿用于臨床與診斷。
說明書下載:m6A RNA Methylation Quantification Kit(Colorimetric).pdf